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  1. doofaloofa is offline

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    Posted On:
    9/09/2011 3:59pm


     Style: mma

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    Hell yeah! Hell no!
    Are you hitting on me?
  2. jubei33 is offline
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    Posted On:
    9/09/2011 4:48pm


     Style: Boxing, Solar Ray Attack

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    Hell yeah! Hell no!
    Quote Originally Posted by doofaloofa View Post
    Are you hitting on me?
    Well, you're posting pictures of asses in my thread. Most people would think that thought's ass backwards. But look, if you really want to 'get into my pants' I'm a fussy, expensive date. You're going to have to raise the stakes in the cancer dept--viz. some insightful knowledge into new modes of clinical therapy, diagnosis, etc...

    So more to the point, selective targeting of cancer cells. This is kind of the holy grail of cancer chemotherapy. Time will tell, however, if this is a viable therapy in vivo. (You can read most of these at the public library of science for free, by the way.)

    miR-24 Regulates Apoptosis by Targeting the Open Reading Frame (ORF) Region of FAF1 in Cancer Cells

    Abstract
    Background: microRNAs (miRNAs) are small noncoding RNAs that regulate cognate mRNAs at the post-transcriptional stage. Several studies have shown that miRNAs modulate gene expression in mammalian cells by base pairing tocomplementary sites in the 39-untranslated region (39-UTR) of the target mRNAs.

    Methodology/Principal Findings: In the present study, miR-24 was found to target fas associated factor 1(FAF1) by binding to its amino acid coding sequence (CDS) region, thereby regulating apoptosis in DU-145 cells. This result supports an augmented model whereby animal miRNAs can exercise their effects through binding to the CDS region of the target mRNA. Transfection of miR-24 antisense oligonucleotide (miR-24-ASO) also induced apoptosis in HGC-27, MGC-803 and
    HeLa cells.

    Conclusions/Significance: We found that miR-24 regulates apoptosis by targeting FAF1 in cancer cells. These findings suggest that miR-24 could be an effective drug target for treatment of hormone-insensitive prostate cancer or other types of cancers. Future work may further develop miR-24 for therapeutic applications in cancer biology.
    Qin W, Shi Y, Zhao B, Yao C, Jin L, et al. (2010) miR-24 Regulates Apoptosis by Targeting the Open Reading Frame (ORF) Region of FAF1 in Cancer Cells. PLoS ONE 5(2): e9429. doi:10.1371/journal.pone.0009429
    Last edited by jubei33; 9/09/2011 4:49pm at . Reason: cite
    http://woodwardswhiskey.wordpress.com/

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  3. doofaloofa is offline

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    Posted On:
    9/10/2011 2:40am


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    Hell yeah! Hell no!
    i'm just scared. Scientist types have hurt me before
    They start of all 'selective targeting of cancer cells' and it ends 'I'm biffing that cute lab technician...sorry'
  4. jubei33 is offline
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    Posted On:
    9/10/2011 6:01am


     Style: Boxing, Solar Ray Attack

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    Hell yeah! Hell no!
    Oh, is that what this is---Are you trying to get a job?

    ...

    What are your skills?

    Do you have any experience with HPLC, particularly with high throughput systems/isolations from natural sources. We were looking at seaweed recently, or more specifically the bacteria that grow on it. We want to advance out ability to culture and entice certain kinds to produce new antibiotics and cancer drugs.

    or there's always the bees, that's a project that's always looking for people.
    http://woodwardswhiskey.wordpress.com/

    He was punching him like the collective karmic debt he'd accrued was coming to collections, mostly on his face.
  5. doofaloofa is offline

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    Posted On:
    9/10/2011 7:14am


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    Hell yeah! Hell no!
    Skills...me strong like bull

    The only high throughput systems I am familiar with are pigs and babies

    I am a former bee keeper

    And to get things straight I am totaly opposed to sexual harrasment in the work place
  6. jubei33 is offline
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    Posted On:
    9/10/2011 3:59pm


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    Bee keeper, huh?
    Any knowledge of handling and shipping dangerous cargoes, including plants and animals?

    How about bees whose stings cause semi-permanent diarrhea?

    We are also working on taking taxanes from species of yew trees. As a side project, we are working on transplanting these species, acclimating them to other varied environments. Taxanes, as you know, are under investigation in the treatment of many kinds of cancer. These are difficult to synthesize cheaply, because of how many chiral centers they generally contain, so a route would be increasing the available crop. This is a weak compromise, as the real money is in finding out how these chemicals inhibit the mitotic spindle and replicate that effect with a cheaper to produce chemical skeleton.
    Last edited by jubei33; 9/10/2011 4:14pm at .
    http://woodwardswhiskey.wordpress.com/

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  7. doofaloofa is offline

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    Posted On:
    9/10/2011 4:31pm


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    Well I have transported hives a good bit, but never very far.

    bee stings that cause diarrhea...nice. I got stung by a wasp yesterday and thats sore enough thank you!

    As for Taxanes, are they in the nuts (realy specialised cones). I understand that nuts are poisonose, but the red flesh that surrounds them is quite tasty. I have a few european yews (T baccata) but they are only young. Very active in folklore and magic, the yew tree, and bows of coarse. I wasn't aware of thier prospects of curing cancer

    How do you like this hypothesis. Some kind of cancer type cellular pathology creating the kind of zombie like symptoms we see in movies etc. Maybe with a viral vector...food for thought!
  8. jubei33 is offline
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    Posted On:
    9/10/2011 5:20pm


     Style: Boxing, Solar Ray Attack

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    Quote Originally Posted by doofaloofa View Post
    Well I have transported hives a good bit, but never very far.

    bee stings that cause diarrhea...nice. I got stung by a wasp yesterday and thats sore enough thank you!

    As for Taxanes, are they in the nuts (realy specialised cones). I understand that nuts are poisonose, but the red flesh that surrounds them is quite tasty. I have a few european yews (T baccata) but they are only young. Very active in folklore and magic, the yew tree, and bows of coarse. I wasn't aware of thier prospects of curing cancer

    How do you like this hypothesis. Some kind of cancer type cellular pathology creating the kind of zombie like symptoms we see in movies etc. Maybe with a viral vector...food for thought!
    I'm not sure about the nuts, but the taxanes are usually extracted from the bark of the tree. Taxol, the first of these compounds to be noticed, was derived from the pacific yew. The problem was there weren't enough of them to mass produce the chemical without driving them into extinction. Nobody wants that, as there goes your source of not just one, but probably many other possible compounds. And there is the bow and arrow lobby that always wants its share too.

    Fun as they might be, the problem I always with zombies is where they're getting their energy from. So they eat people, but they don't need to eat and they keep going. Sometimes you see them running full tilt in a movie, busting after the lead role or his mian love interest or some other douchebag..or they walk and walk and walk.

    But as a viral vector, you have to think that transmittance is important and if the crop of available hosts is killed off, then there goes the viral epidemic, not enough hosts. Also, now lets say that this dude was a real couch potato, were talking total fatass, right? and he gets infected, there's no way that dude's muscles are going to be able to handle the marathon running zombie style. If he couldn't do it before, how would a viral infection enable him to compete in that way. It must be adrenaline, then, right, the adrenaline rush. So, if we have chemical systems running in the background, then we also must have energy and they must get their energy from somewhere. If its fat or the muscle then that's limited to how much they can acquire + how much store they have on them, which is not really all that much. I mean the liver only stores a few thousand calories on hand, and a body can only really store a limited amount of fat and maintain mobility.....

    not all that scary. Now, diarrhea bees...
    http://woodwardswhiskey.wordpress.com/

    He was punching him like the collective karmic debt he'd accrued was coming to collections, mostly on his face.
  9. doofaloofa is offline

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    Posted On:
    9/10/2011 5:45pm


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    Quote Originally Posted by jubei33 View Post
    Fun as they might be, the problem I always with zombies is where they're getting their energy from.
    why Chi of coarse!

    killer rabits v's diarrhea bees

  10. jubei33 is offline
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    Posted On:
    9/10/2011 6:23pm


     Style: Boxing, Solar Ray Attack

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    A friend and I once discussed animal gigantism with respect towards engineering. He said: “If you’re going to go to the trouble of making something it must be big, it’s more terrifying.” And he is right to some degree, but this route also creates problems that inherently limit the effect. Larger animals need larger amounts of food and are also more visible and prone to counterattack. A hypothetical mantis the size of a man would displace others of its flock by competition, thus limiting the population size even farther. This is also not mentioning the physical and technical barriers to such a feat. A singular natural oddity, a rarity, is less to fear than several thousand. I believe ordinary things can be just as terrifying given the right circumstances.

    nice leepus.

    More on zombie cancer virus:

    Though several viruses have been implicated in causing cancer (HZV,EBV,etc), the problem with using them as a foil is what a tumor does. generally, the leach up all the available nutrients of a given area, just for the sake of growth. They're so good at it in fact, that generally the inside of these kind of tumors are hypoxic with respect to outer layers of tissue. This is a direct result of the ability of nutrients and oxygen to diffuse through tissues, which is described by fick's law of diffusion. This kind of diffusion is limited by distance, thus the further you are away from a capillary the less nutrients diffuse to you. So for zombies at least, this would be a difficult sell even if framed as 'eating people for more energy'.

    why Chi of coarse!
    You're fired. :)
    Last edited by jubei33; 9/10/2011 6:33pm at .
    http://woodwardswhiskey.wordpress.com/

    He was punching him like the collective karmic debt he'd accrued was coming to collections, mostly on his face.
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